Alterations in the metabolic footprint induced by Mayaro virus infection in cell culture

This study focuses metabolic footprinting of Vero cells infected by Mayaro virus. Nuclear magnetic resonance combined with multivariate analytical methods and tools of pattern recognition shows that metabolic changes can be attributed to the effect of Mayaro virus infection. Vero cells infected by Mayaro and incubated for periods of 2h, 6h and 12h show in each period different variations in the levels of several metabolites such as amino acids, organic acids and carbohydrate. These organic compounds are metabolites involved in the pathway of glycolysis, tricarboxylic acid cycle, pentose phosphate pathway, and the oxidation pathway of fatty acids (via the β-oxidation). This study of footprinting analysis demonstrates the effect of the action of virus in the metabolism of the Vero cells, moreover, these analyses indicate intracellular metabolic state, and contributes to the knowledge of the microorganism influence on cellular metabolism.