Expression profiling of 3 and 24 months mouse skeletal muscle (RNA-seq)

Aging in multicellular organisms is characterized by gradual decline of organ functionality. To study the aging process, we used mRNA sequencing (mRNA-seq) to identify gene expression changes during aging in healthy mice. Skeletal muscle tissues of wild-type mice at 3 months and 24 months of age were collected and mRNA-seq libraries were prepared and sequenced on a HiSeq2500 by single-end sequencing with 100 bp read length. Analysis of the expression profiles of aged skeletal muscle tissues showed decreased mRNA levels of genes function in lipid metabolism, peptidase activity and response to stimulus.多细胞生物的衰老以器官功能的渐进性衰退为特征。为研究衰老过程，我们采用mRNA测序（mRNA-seq）技术，鉴定健康小鼠衰老过程中的基因表达变化。我们采集了3月龄与24月龄野生型小鼠的骨骼肌组织，构建mRNA-seq文库，并在HiSeq2500测序平台上采用100 bp读长的单端测序方式完成测序。对衰老骨骼肌组织的表达谱分析显示，参与脂质代谢、肽酶活性调控及应激应答的基因，其mRNA水平出现下调。