The Drosophila Genetic Reference Panel (DGRP) on locomotor activity across different environmental conditions

In nature, organisms are exposed to variable and occasionally stressful environmental conditions. Responses to diurnal and seasonal fluctuations, such as temperature and food accessibility, involve adaptive behavioral and physiological changes. While much work has been done on understanding the genetic architecture and evolutionary potential of stress tolerance traits under constant thermal conditions, there has been less focus on the quantitative genetic background in variable environments. In this study, we use the Drosophila Genetic Reference Panel (DGRP) to investigate locomotor activity, a key behavioral trait, under variable natural thermal conditions during the summer in a temperate environment. Male flies from 100 DGRP lines were exposed to natural thermal and light conditions in Drosophila activity monitors across three experimental days. We found that activity was highly temperature- and time-dependent and varied between lines both within and between days. Further, we observed..., Drosophila stocks and maintenance
A total of 127 lines of the Drosophila Genetic Reference Panel (DGRP) (doi:10.1101/GR.171546.113.) were obtained from Bloomington Drosophila Stock Center (NIH P40OD018537. The flies were maintained on a standard Drosophila oatmeal-sugar-yeast-agar medium (7 mL) at 20 °C, and a 12:12 h light/dark photoperiod (light on from 08:00a.m. to 08:00p.m.) for two generations before experimental start. The flies were pre-fed with additional dry yeast on the surface of the Drosophila medium before oviposition to generate flies used for the experiments.
Experimental setup
Experimental flies were produced from three replicate vials per line (100 DGRP lines were used). In each replicate vial, 15-20 randomly selected adult flies, comprising both males and females, were permitted to lay eggs for four 12-hour periods, with the flies being transferred to new vials every 12 hours. Within 48 hours of eclosion, flies were sexed under CO2 anesthesia and males were transferred..., , # Data from: The Drosophila Genetic Reference Panel (DGRP) on locomotor activity across different environmental conditions

## Description of the data and file structure

The data set contains individual measurements of a single fly's locomotor activity (summed in bins of 20 min) across 24 hours for three different experimental test days.

The data set contains 9 columns (see description below) and 344,000 rows (corresponding to individual observations). The file is a comma seperated file (*.csv)

`lineID` internal lab individual DGRP ID

`RAL_full` DGRP id

`stock` Bloomingstock line ID

`datetime` indicator for date, and time of day the 20-min bins belong to.

`batch` indicator variable for which of the three experimental test day the observation belong to.

`diff` indicator variable of the 20-min bins ranging from zero to 1460.

`tube` indicator variable of the individual DAM2 glass tubes.

`Monitor` indicator variable of the DAM2 monitor ID.

`act_sum` The sum of activity (number of...