An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis

mRNA quality control mechanisms ensure fidelity of protein translation. An evolutionarily conserved component of the quality control machinery, Dom34/Pelota (Pelo), rescues stalled ribosomes. Here we show that Pelo is required for mammalian epidermal homeostasis. Our study reveals a novel role for the ribosome-rescue machinery in mammalian tissue homeostasis and an unanticipated specificity in its impact on different stem cell populations. Overall design: RNA-seq of mouse epidermal stem cell subpopulations (Lrig1+, Lgr5+ and Lgr6+) in WT and Pelota KO mice. We have triplicates for each experimental condition.