BSA-seq analysis of the small-grain mutant R140 and the mid-double 11 extreme populations

In order to analyse the genetic basis of the phenotypic differences in grain size between the small-grain mutant R140 and the conventional variety Zhongshuang11, we constructed extreme segregant populations (30 plants each of the large-grain and small-grain types) after crossing the two, and located the key candidate intervals through Bulked Segregant Analysis (BSA) combined with high-throughput sequencing (BSA-seq).