RNA-seq of human RNA contaminated with different amounts of mouse gDNA to quantify the impact of gDNA contamination in prime-seq. RNA-seq of human RNA contaminated with different amounts of mouse gDNA to quantify the impact of gDNA contamination in prime-seq

The impact of gDNA contamination on RNA-seq experiments performed with the prime-seq method was assessed by introducting artifical contamination using mouse gDNA this enables us to identify contamination by species specific assignment of mapped reads.