Assessment of epigenetic variation in human iPS cells-Medip

n order to progress human induced pluripotent stem cells (hiPSCs) towards the clinic, several outstanding questions must be addressed. It is possible to reprogram different somatic cell types into hiPSCs and from studies in the mouse, it appears that an epigenetic memory of the starting cell type is carried over to hiPSCs. However a comprehensive comparative study of the characteristics of these hiPSCs has been missing from the literature. Importantly studies which aimed to address these aspects of hiPSCs have used cells from different patients. In order to avoid this important confounding variable and to keep the genetic background constant, tissue samples were procured from the patients and reprogrammed to iPS cells. The methylation status of these iPS cells will be compared.Protocol:Primary cell cultures were generated and reprogrammed to iPS cells. DNA was extracted and immunoprecipitated using anti-methyl cytosine and anti-hydroxymethyl cytosine antibodies.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

为推动人类诱导多能干细胞（hiPSCs）的临床应用，必须解决一系列关键问题。目前，将不同类型的体细胞重编程为hiPSCs已成为可能，而根据对小鼠的研究，原始细胞类型的表观遗传记忆似乎也被携带至hiPSCs中。然而，关于这些hiPSCs特征的全面比较研究在文献中尚属缺失。值得注意的是，旨在解决hiPSCs这些方面的研究均使用了来自不同患者的细胞。为了避免这一重要混杂变量并保持遗传背景的一致性，我们从患者处采集了组织样本并将其重编程为iPS细胞。这些iPS细胞的甲基化状态将被比较。研究方法：首先生成和重编程为iPS细胞的原代细胞培养物。随后，使用抗甲基胞嘧啶和抗羟甲基胞嘧啶抗体提取DNA并进行免疫沉淀。这些数据是预发表数据的一部分。关于惠康信托桑格研究所（Wellcome Trust Sanger Institute）共享的预发表数据的适当使用（包括任何发表禁令的细节），请参阅http://www.sanger.ac.uk/datasharing/。