DataSheet_4_Down-regulated expression of CDK5RAP3 and UFM1 suggests a poor prognosis in gastric cancer patients.pdf

PurposeThe relationship between the CDK5RAP3 and UFM1 expression and the prolonged outcomes of patients who underwent gastric cancer (GC) surgery was investigated.MethodsSingle-sample gene set enrichment analysis (ssGSEA), unsupervised clustering and other methods were used to verify the relationship between CDK5RAP3 and UFM1 in GC through public databases. Additionally, CDK5RAP3 and UFM1 expression in cancerous and paracancerous tissues of GC was analysed in the context of patient prognosis.ResultsCDK5RAP3 and UFM1 expression was downregulated synchronously, the interaction was observed between the two proteins, and UFM1 and CDK5RAP3 expression was found to be inversely associated to AKT pathway activation. Prognostic analysis showed that the prognosis is poorer for low CDK5RAP3 and UFM1 patients, than for high CDK5RAP3 and/or UFM1 (p

本研究旨在探究CDK5RAP3与UFM1表达与接受胃癌（GC）手术患者长期预后之间的关系。研究方法包括使用单样本基因集富集分析（ssGSEA）、无监督聚类等方法，通过公共数据库验证CDK5RAP3与UFM1在GC中的关联性。此外，在分析患者预后时，对GC癌组织和癌旁组织中CDK5RAP3和UFM1的表达进行了分析。结果显示，CDK5RAP3和UFM1表达同步下调，观察到两种蛋白之间的相互作用，并且发现UFM1与CDK5RAP3的表达与AKT通路激活呈负相关。预后分析表明，与高CDK5RAP3和/或UFM1患者相比，低CDK5RAP3和UFM1患者的预后更差（p值待补充）。