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Interactions between Yeast Proteins and Rsp5 Ubiquitin Ligase

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NIAID Data Ecosystem2026-03-07 收录
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To identify substrates of the ubiquitinating E3 enzyme Rsp5 we applied purified Rsp5 to duplicate protein arrays. The Rsp proteins were expressed as fusion proteins to GST. We used as a control Ubr1, a RING domain containing E3 ligase We analyzed Rsp5 from S.cerevisiae on duplicate arrays, with four control chips, two without Rsp5 and two with Ubr1.

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2012-03-20
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