Characterisation and fluorescence intensity of tryptophan mutant proteins.
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a(M−1s−1 (×105).bMelting temperature (Tm in °C) is defined as the inflection point of the thermal unfolding.cPercentage (%) change in fluorescence intensity calculated at 340 nm. Abbreviations are as follow (NC) = no change; (∼) = not determined; (U) = uninterpretable. All experiments are the average of three independent replicates.
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2015-12-02
