Identification of altered blood microRNAs in a rat model of Parkinson’s disease by Custom "Brain Specific miRNA" Open Array Real-time PCRpanel

12 week male wistar rats were exposed with rotenone (2.5mg/kg b.wt.) and control rats exposed with vehicle (corn oil) intraperetonially for two months. Animals were sacrifised at the end of the experiment and blood was isolated and stored at -80°C in RNALater until further analysis.RNA (including small RNAs) was extracted by Ribopure Blood RNA Isolation Kit by Thermo Fisher as suggested protocol by manufacturer. A custom Taqman OpenArray panel was used to profile the miRNAs expression. RNA isolated from control and rotenone exposed group blood samples were quantified and perfomed reverse transcription reaction using custom primer pool for cDNA synthesis. Further preamplification of CDNA sample was also performed using preamplification primer pool. The preamplification products then diluted in TE buffer and mixed with Taqman Open Array universal master mix and run the Open array.