Sequence characteristics of undiluted and serially diluted saliva (1 individual), isolated with the Agowa extraction method.

A clear increase in unique sequences, unclassified bacteria and Shannon diversity index was seen in dilution 3 (105 bacteria/ml). Undiluted = undiluted saliva, 1 = dilution 1, 107 bacteria/ml, 2 = dilution 2, 106 bacteria/ml and 3 = dilution 3, 105 bacteria/ml.*Using a moving window 50 bp long to check that the average quality score over the region does not drop below 35 (using the qual file).§The coverage calculator returns Good's coverage for an OTU definition.