RIP1 inhibition protects TMJ from stress-induced TMJ OA

A compressive mechanical force-induced-TMJ OA rat model was used in the study. Lenti-virus targeting RIP1 was constructed in vitro and injected into the TMJ. Hematoxylin and eosin staining, TUNEL staining and micro-computed tomography were used to assess histological and morphological changes in the mandibular condyle. Immunohistochemistry, Western Blot and real-time PCR were performed to determine the changes of inflammatory, apoptotic and necroptotic markers.

本研究采用了一种由压缩性机械力诱导的颞下颌关节骨关节炎（TMJ OA）大鼠模型。在体外构建了靶向RIP1的 Lentivirus，并将其注射至颞下颌关节。通过苏木精-伊红染色、TUNEL染色及显微计算机断层扫描技术对下颌髁的病理和形态学变化进行评估。此外，通过免疫组化、蛋白质印迹和实时定量PCR技术检测炎症、凋亡和坏死性标记物的变化。