Expression data from Saccharomyces cerevisiae upon honokiol treatment. Saccharomyces cerevisiae

Honokiol (HNK), one of the main medicinal components in Magnolia officinalis, possesses antimicrobial activity against a variety of pathogenic bacteria and fungi.S. cerevisiae is a model eukaryote used for investigating the cellular and molecular mechanisms of anti-fungal drugs. To explore the molecular mechanism of its anti-fungal activity, we determined the effects of HNK on the mRNA expression profile of Saccharomyces cerevisiae using a DNA microarray approach. Overall design: Wild-type S. cerevisiae (BY4741) cells were treated with 12 μg/mL HNK for 2 h (or an equal volume of ethanol for controls) and for RNA extraction and hybridization on Affymetrix microarrays. Biological replicates: 2 control replicates, 2 HNK treated replicates.