Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (scRNA-Seq)

To test the function of LEENE in leene-KO mice and delineate the contribution of LEENE RNA in the ischemic response, we supplemented the leene-KO mice with human LEENE RNA and performed single cell RNA-seq. The transcriptome of the EC-enriched fractions in the ischemic muscles was profiled at the single cell level. The leene-KO mice with Hindlimb ischemia (HLI) were supplemented with adenovirus overexpressing GFP or human LEENE via muscle injection. The ischemic muscles were dissected, endothelial cells were isolated and enriched by CD144 beads. Single cell RNA-seq was performed by using the 10XGenomics 3’ gene expression assay.