Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [ACA]

We measued IgG autoantibodies associated with Connective Tissue Diseases (CTDs) and Anti-Cytokine Antibodies (ACA) in SLE and healthy controls who received the BNT162b2 vaccine. We constructed protein arrays by first coupling recombinant protein antigens to uniquely fluorescent carboxylated magnetic beads. We then combined the beads into two separate arrays that were used to probe patient SLE serum samples, healthy control samples, and autoimmune prototype samples. A Luminex FLEXMAP 3D® system was used to quantitatively measure autoantibody reactivity in each sample in units of mean flourescent intensity (MFI).