RPE1-E150-Benchmarking

Processed dataset of all essential gene CRISPRi perturbations within RPE-1 cells (benchmarking overloading) from: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. PMID: 39131349; PMCID: PMC11312553. in press at Nature Genetics Raw fastq files available at SRA: PRJNA1108254 This record contains aggregated, quality control filtered, and processed outputs downstream of cellranger including. 1) guide umis per cell: rpe1_e150_maxol_exp_norm_crispri_aggr_total_guide_umis.h5 2) final qc filtered population with multiplets: rpe1_e150_maxol_crispri_scanpy_from_cellranger_aggr_singlets_and_multiplets_5umi_cellranger_guide_calls.h5ad 3) regression model benchmarking inputs: RPE1_E150_all_genes.h5ad, rpe1_e150_maxol_crispri_full_design_matrix_guide_identity.csv