Lipid overload-induced R Loops formed by m6A-modified circTACC3

To further elucidate the pathological characteristics of circTACC3-R loops in the HCC genome, circR loops were enriched by DRIP from lipid overload-induced HCC cells. Based on this premise, we devised an oligonucleotide probe specifically targeting the BSJ region of circTACC3, and used a ChIRP assay to selectively enrich the circR loops formed by endogenous circTACC3, named “DRIP-ChIRP”, by which specific enrichment of circTACC3 was detected within global circR loops and circTACC3-R loops Overall design: circR loops were enriched by DRIP from (S9.6 Antibody) lipid overload-induced HepG2 cells. Based on this premise, we devised an oligonucleotide probe specifically targeting the BSJ region of circTACC3, and used a ChIRP assay to selectively enrich the circR loops formed by endogenous circTACC, by which specific enrichment of circTACC3 was detected within global circR loops and circTACC3-R loops