Inhibition of H3K9me2-specific histone methyltransferase, G9a/GLP, reveals transcripts regulated by the establishment of H3K9me2 in hematopoietic stem and progenitor cells

We have performed RNA-seq from DMSO control treated, G9a/GLP inhibitor (UNC0638) treated, and DNA-demethylating agent (Decitabine) treated CD34+ hematopoietic stem and progenitor cells. This experiment was performed to identify transcripts that were uniquely affected by the loss of H3K9me2 in hematopoietic stem and progenitor cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CD34+ hematopoietic stem and progenitor cells treated with DMSO, UNC0638, or Decitabine