Table_7_Cross-Sectional Analysis of the Correlation Between Daily Nutrient Intake Assessed by 7-Day Food Records and Biomarkers of Dietary Intake Among Participants of the NU-AGE Study.pdf

Methods for measuring diet composition and quantifying nutrient intake with sufficient validity are essential to study the association between nutrition and health outcomes and risk of diseases. 7-day food records provides a quantification of food actually and currently consumed and is interesting for its use in intervention studies to monitor diet in a short-term period and to guide participants toward changing their intakes. The objective of this study is to analyze the correlation/association between the daily intake of selected nutrients (collected by a 7-day food records plus a mineral/vitamin supplementation questionnaire) and estimates of energy expenditure as well as blood and urine biomarkers of dietary intakes in 1,140 healthy elderly subjects (65–79 years) at baseline of the NU-AGE intervention study (NCT01754012, clinicaltrials.gov). The results show that: the daily intake of energy correlated significantly with predicted total energy expenditure (pTEE) (ρ = 0.459, p < 0.001, and q < 0.001); protein intake correlated significantly with the ratio of 24 h urinary urea to creatinine excretion (ρ = 0.143 for total protein intake, ρ = 0.296 for animal protein intake, and ρ = 0.359 for protein intake/body weight, p < 0.001 and q < 0.001 for each correlation); vitamin B12 and folate intakes correlated significantly with their serum concentrations (ρ = 0.151 and ρ = 0.363, respectively; p < 0.001 and q < 0.001 for each correlation); sodium and potassium intakes correlated significantly with their 24 h urinary excretion (ρ = 0.298 and ρ = 0.123, respectively; p < 0.001 and q < 0.001 for each correlation); vitamin B12 and folate intakes were negatively associated with plasma homocysteine measure (p = 0.001 and p = 0.004, respectively); stratifying subjects by gender, the correlations between energy intake and pTEE and between potassium intake and its 24 h urinary excretion lost their significance in women. Even if the plasma and urinary levels of these nutrients depend on several factors, the significant correlations between daily reported intake of nutrients (protein, vitamin B12, folate, and sodium) and their blood/urinary markers confirmed that the 7-day food records (plus a supplementation questionnaire) provides reliable data to evaluate short-term current dietary intake in European elderly subjects and it can be exploited to guide and monitor NU-AGE participants through the shift of their diet according NU-AGE recommendations.

衡量膳食组成及量化营养摄入的有效方法对于研究营养与健康结果及疾病风险之间的关联至关重要。七日食物记录能够量化实际及当前摄入的食物量，其在干预研究中用于短期监测膳食变化并指导参与者调整摄入量方面具有重要意义。本研究的目的是分析所选营养素（通过七日食物记录及矿物质/维生素补充问卷收集）的每日摄入量与1,140名健康老年受试者（年龄介于65至79岁）在NU-AGE干预研究（NCT01754012，临床研究网）基线阶段的能量消耗估算以及血液和尿液中的膳食摄入生物标志物之间的相关性/关联。研究结果显示：每日能量摄入与预测的总能量消耗（pTEE）（ρ = 0.459，p < 0.001，q < 0.001）显著相关；蛋白质摄入与24小时尿液中尿素与肌酐排泄比（总蛋白质摄入ρ = 0.143，动物蛋白质摄入ρ = 0.296，蛋白质摄入/体重ρ = 0.359，每项相关性p < 0.001，q < 0.001）显著相关；维生素B12和叶酸摄入与它们的血清浓度（维生素B12 ρ = 0.151，叶酸ρ = 0.363；每项相关性p < 0.001，q < 0.001）显著相关；钠和钾摄入与它们的24小时尿液排泄（钠ρ = 0.298，钾ρ = 0.123；每项相关性p < 0.001，q < 0.001）显著相关；维生素B12和叶酸摄入与血浆同型半胱氨酸水平呈负相关（维生素B12 p = 0.001，叶酸p = 0.004）；按性别分层后，能量摄入与pTEE以及钾摄入与其24小时尿液排泄之间的相关性在女性中失去了显著性。尽管这些营养素的血浆和尿液水平受多种因素影响，但每日报告的蛋白质、维生素B12、叶酸和钠摄入量与它们的血液/尿液标志物之间的显著相关性证实了七日食物记录（加上补充问卷）为评估欧洲老年受试者的短期当前膳食摄入提供了可靠数据，并可被利用以根据NU-AGE建议指导并监测NU-AGE参与者膳食的转变。