George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:15789, Dendraster excentricus. CIL. Dataset

One cell within a 16-cell sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 25 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells 10 min before time 00:00. Although the aster regrows slightly, the cortex is ∼20 µm away from the nearest spindle microtubule ends; nevertheless, cytokinesis completes accurately. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video correspond to Fig 4C and second segment of Video 4 in J Cell Biol. 2009 Dec 14;187(6):831-45.

在16细胞海胆胚胎中的一个细胞中，表达着与微管结合蛋白的ensconsin结构域融合的三个串联的绿色荧光蛋白（GFPs）（Faire等，1999年）。该胚胎在00:00时间点前10分钟用25 µM的三环己基胺（TSA）处理，TSA是一种抑制HDAC6（一种微管去乙酰化酶）的抑制剂，它能选择性地破坏哺乳细胞中的动态微管。尽管星状结构略有再生，但细胞皮层距离最近的纺锤体微管末端约20 µm；尽管如此，细胞分裂仍能准确完成。使用安装在复合显微镜（E800；尼康）上的激光扫描共聚焦显微镜（Radiance 2000；Bio-Rad实验室）和40× 1.3 NA Plan-Fluor油镜头对海胆胚胎进行了成像。室温保持在14–17°C。视频对应于J Cell Biol. 2009年12月14日；187(6):831-45的图4C和视频4的第二部分。