The effects of input RNA quantity on cDNA-capture sequence, using commercially prepared human RNA. Homo sapiens

To assess the consequence of a lower quantity of input material on the quality of the resulting sequence data, RNA sequencing combined with exome capture sequencing - a technique we refer to as cDNA-Capture - was performed on varying amounts (60, 50, 10, 2 and 0.8ng) of MVP Disease Match Set Human Adult Colon RNA derived from normal and adenocarcinoma margin (Stratagene catalog number 540077). Each sample was sequenced in triplicate.