遇见数据集

Raw, normalised, preprocessed and segmented IMC images

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Figshare2025-05-08 更新2026-04-08 收录
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Output images generated by the automated image processing software SIMPLI (https://github.com/ciccalab/SIMPLI - https://doi.org/10.1038/s41467-022-28470-x).<b>Raw</b>: original images generated by the IMC acquisition. <b>Normalised</b>: raw images individually processed with 99th percentile normalisation by SIMPLI.<b>Preprocesed</b>: normalised images adjusted by threshold and masking by SIMPLI using a pre-defined Cell Profilier pipeline. Images generated in this step are used for the consecutive pixel-based (area_measurements.csv) and cell-based (clustered_cells.csv) analyses.<b>Segmentation</b>: output files from segmentation of the DNA1 channel performed by SIMPLI using StarDist. This step is necessary to identify all areas corresponding to individual nuclei and to proceed with cell-based analyses (clustered_cells.csv).<br>

提供机构:
Caramello, Alessia
创建时间:
2025-04-02
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