Sex Lethal is recruited to chromatin by RNA polymerase III [DamID-seq]

We discovered that the splicing factor Sex lethal (Sxl) also associates strongly with chromatin and that this association is not linked to its RNA-binidng capacity, nor its N- or C-terminal domains. We also found that Sxl is recruited to chromatin by the RNA polymerase III subunt Polr3E. Profiling of Sex lethal (Sxl) and Polr3E DNA binding in larval neurons of the Drosophila brain. Also includes binding of Sxl mutants and Sxl in a Polr3E RNAi background.