Assemblies, associated annotation files, and analysis source data of Platanus x acerifloia genome

Platanus × acerifolia (London plane; Platanaceae) is a major ornamental tree used worldwide. Platanaceae is one of the last early-diverging eudicot families without a complete nuclear genome assembly. Here, we assembled a high-quality, chromosome-level reference genome for P. × acerifolia., Genome and transcriptome sequencing
The extracted genomic DNA met the requirement for both Illumina (San Diego, CA, USA) sequencing and PacBio High Fidelity (HiFi; Covaris, Massachusetts, USA) sequencing (Supplementary Note 2.2). For Illumina sequencing of the P. × acerifolia genome, a 350-bp library was constructed and sequenced on the Illumina NovaSeq platform. The approximately 10-kb SMRTbellTM libraries were constructed for single-molecule sequencing with PacBio; the data generated were used to assemble the highly heterogeneous P. × acerifolia polyploid genome. The HiFi reads were sequenced using the PacBio Sequel II system following PacBioTM standard protocol. To anchor genome scaffolds onto chromosomes, we also constructed a Hi-C library and obtained sequence data via the MGI-SEQ 2000 platform (Wuhan, Hubei, China). In brief, fresh leaves were fixed with a nuclei isolation buffer containing 2% formaldehyde following previously reported methods. The DNA was digested with DpnII. Bio..., , # Assemblies, associated annotation files, and analysis source data of Platanus x acerifloia genome

[https://doi.org/10.5061/dryad.j6q573nm9](https://doi.org/10.5061/dryad.j6q573nm9)

## Description of the data and file structure

This is the genome assembly, annotation, and source file of *Platanus* x *acerifloia*

| File Name | Description |
| ------------------------------------------ | ------------------------------------------------------------------ |
| Platanus\_acerifolia\_Group1\_genome.fasta | Pseudo-haplotype genome sequence file |
| Platanus\_acerifolia\_Group1.gff | Pseudo-haplotype genome annotation file |
| Platanus\_acerifolia\_Group1\_prot.fasta | Pseudo-haplotype AA sequence file |
| Platanus\_acerifolia\_Group1\_CDS.fasta | Pseudo-haplotype CDS sequence file ...