Identification of potential Rsf1 promoter-interacting proteins in rice (Oryza sativa Nipponbare) roots during nematode infection

We performed DNA-pull down experiments using biotin-labeled and unlabeled promoter sequences of Rsf1, a susceptibility factor in rice against root-knot nematode infection, to capture interacting nuclear proteins from rice roots at different time points post-infection (0, 1, and 3 days). Captured proteins were analyzed by LC-MS/MS to identify potential transcriptional regulators or binding partners of the Rsf1 promoter.