Dominguez sleep deprived flies

The sleep deprivation experiment was done in wild flies or in pumilio knockdown expression (using RNA interference). Pumilio seems critical for the homeostatic response to sleep deprivation.When wild flies are sleep deprived, they respond by sleeping more after the deprivation stimulus is removed. Pumilio RNAi (expression knockdown) sleep less after sleep deprivation. Analyzing these samples, we can see if sleep deprivation leads to the disregulation of intestinal microbes, and if pumilio plays a role on this regulation.