RNA-seq profiling of Pleurotus ostreatus exposed to BBP with or without cytochrome P450 inhibition

This study provides transcriptomic insights into the degradation of butyl benzyl phthalate (BBP) by the white-rot fungus Pleurotus ostreatus. RNA-seq was conducted for samples treated with BBP alone or in combination with the cytochrome P450 inhibitor piperonyl butoxide (PB). The aim was to investigate the role of fungal enzymes in BBP metabolism. Differentially expressed genes related to xenobiotic degradation, oxidative stress, and P450 activity were identified and analyzed. Pleurotus ostreatus GEMB-PO1 was cultured under three conditions: control (CK), BBP treatment (50 mg/L), and BBP plus piperonyl butoxide (BBP+PB, 50 mg/L BBP with 1 mM PB). Fungal mycelia were harvested after 3 days for RNA extraction and sequencing. Three biological replicates were included for each group.