Measuring RBC subpopulations using archived automated hematology analyzer reports

In preliminary studies, we demonstrated that an on-line application of Gauss’ area formula (SketchAndCalc™) measured percentage areas of 20 segments under a computer-generated normal distribution curve (−3.0 standard deviations (SD) to +3.0 SD) with accuracy and precision (Pearson's correlation of measured areas with corresponding theoretical areas r[20]=0.9962 (p<0.0001)). Thus, we used SketchAndCalc™ to quantify percentages of microcytes (50-80 fL) and macrocytes (110-200 fL) in archived AHA histogram images in women with previously untreated iron-deficiency anemia (IDA) and previously untreated hemochromatosis.