ConsensusPrime - a bioinformatic pipeline for efficient consensus primer design. Detection of various resistance and virulence factors in MRSA - a case study.

We present a use case for developing a set of primers and probes to identify antibiotic resistance and virulence genes in Staphylococcus aureus using the ConsensusPrime pipeline. Extensive qPCR experiments with several S. aureus strains confirm the exceptional quality of the primers designed using the pipeline. By improving the quality of the input sequences, the pipeline ensures high-quality primers and probes, which should be the basis of molecular assays. The ConsensusPrime pipeline was developed to streamline and simplify the input data curation and consensus primer design process.