Cryptic isoprene emission of soybeans under wounding and high temperature

Isoprene is the most abundant non-methane biogenic hydrocarbon emitted by some plants, mostly trees. It plays a critical role in atmospheric chemistry by contributing to ozone and aerosol formation. Isoprene also benefits plants, particularly under stress, through its signaling roles. Legume crops like soybean were thought to have evolutionarily lost isoprene synthase (ISPS) and are typically considered non-emitters. Here we report that damage to soybean leaves by wounding or burning triggered a burst of isoprene emission from undamaged parts of the leaves. In silico analysis identified intact ISPS genes in the soybean genome, with features similar to known ISPSs. Protein made from these gene sequences catalyzed isoprene production in the presence of dimethylallyl diphosphate. Isoprene emission in soybeans was linked to reduced photosynthesis rates and stomatal conductance. Metabolomic analysis showed that leaf damage caused a surge in glyceraldehyde 3-phosphate and pyruvate levels..., , # Cryptic isoprene emission of soybeans

Dataset DOI: [10.5061/dryad.51c59zwkh](10.5061/dryad.51c59zwkh)

## Description of the data and file structure

To understand the cryoptic isoprene emission nature of crop plant soybean. 

### Files and variables

#### File: Raw\_data\_Mostofa\_et\_al.\_2025.xlsx

**Description:** We measured changes in isoprene emission, photosynthesis and related metabolites, and functional validation of soybean isoprene synthase proteins. 

##### Variables

Figure 2: Expression and purification of recombinant soybean isoprene synthases (ISPSs) and in-vitro characterization of purified ISPSs. (a) Isoprene synthase (ISPS) genes from eucalyptus (Eg_ISPS) and soybean (TPS8 and TPS23) were cloned to an IPTG-inducible expression plasmid followed by expression in E. coli BL21, and purification using a Strep affinity column. (b) Diagram of Strep-tagged ISPS genes and chromatogram of ISPS detected by Fast protein liquid chromatography (FPLC). Strep affinity tag (green ...,