Genome-wide SNP identification and association mapping for seed mineral concentration in Mung bean (Vigna radiata L.)

Mung bean (Vigna radiata L.) quality is dependent on seed chemical composition, which in turn determines the benefits of mung bean consumption for human health. While rich in a range of nutritional components, such as protein, macro- and micro- nutrients, carbohydrates and vitamins, mung bean remains less well studied than other legume crops. Mung bean genomics and genetic resources are relatively sparse. To further improve nutritional levels of mung bean grain requires genome-wide marker system tools. The objectives of this research were to develop these tools and conduct nutrient analysis in order to 1) identify single nucleotide polymorphisms (SNPs) using genotyping by sequencing (GBS) and to 2) perform genome-wide association studies (GWAS) for levels of calcium, iron, potassium, manganese, phosphorous, sulfur, and zinc in mung bean grain produced over two years of field experiment. A total of 112 GWAS models were explored using 6,486 high quality SNPs discovered in 92 cultivated mu...