RNA Sequencing Facilitates Quantitative Analysis of Adenovirus-treated Eed Floxed Mouse Mesenchymal Stem Cells Gene Expression

Purpose: RNA sequencing has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare Cre-treated versus YFP-treated mouse mesenchymal stem cells regarding gene expression. Mesenchymal miRNA profiles of 10-day-old Eed floxed calvaria infected with YFP and Adenovirus-CRE cells were generated by deep sequencing, in triplicate, using BGISEQ-500. The sequence reads that passed quality filters were mapped using HISAT and Bowtie2. Isoform expression levels were analyzed using RSEM. Overall design: Calvarial mRNA profiles of 10-day old YFP and Cre treated mice with floxed Eed genes were generated by deep sequencing, in triplicate, using BGISEQ-500.