UUATAC Data

Current understanding of regulatory sequences in the vertebrate genome are still incomplete. By introducing an adapter switching strategy, we developed an ultra-throughput ultra-sensitivity single-nucleus ATAC-seq (UU-ATAC-seq) protocol that enables construction of high-quality chromatin accessibility landscape from one species in a one-day experiment. Using UU-ATAC-seq, we mapped open chromatin regions for five representative vertebrate species at the landscape scale.<b>File Extension Specification</b><b>proj_MouseFemale.rds:</b> The project of mouse (female) after running a standard ArchR pipeline, containing the metadata derived from fragment files (e.g., TSS enrichment score, unique fragments), LSI dimensionality reduction information, and etc.proj_MouseMale.rds, proj_Chicken.rds, proj_Gecko.rds, proj_Axolotl.rds, proj_Zebrafish.rds are projects for the mouse (male), chicken, gecko, axolotl and zebrafish, respectively.<b>Cell_info_5species.txt:</b> The cell annotation including barcode, tissue source, lineage, UMAP coordinates, major cell type and cell label for five species. Sheets were entitled as species names.<b>AmexG_processed.fa.gz:</b> The DNA sequences of axolotl genome (AmexG_v6.0-DD) after segmentation.<b>AmexG_processed.gtf.gz:</b> The gene annotation file of axolotl genes (AmexG_v6.0-DD) after segmentation.<b>CUTTag_data.tar:</b> CUT&amp;Tag sequencing data for murine adrenal and pituitary glands, profiling histone modifications (H3K4me1, H3K4me3, H3K27ac) and the transcription factor CTCF.<br>