Low-cost, low-bias and low-input RNA-seq with High Experimental Verifiability based on Semiconductor Sequencing. Homo sapiens

We developed a new method on sequencing low-input RNA. This method shows much low-bias with the advantage of semiconductor while competing with smart-seq2. In order to analyze the low-input RNA datasets sensitively, we also develop FANSe2splice with high experimental verification rate as the analysis tool in our method. Overall design: The RNA of HBE and A549 were sequenced in our method. And smart-seq2 was also examined in HBE RNA.