FoxO4 ChIP-Seq profiles of WT differentiated TH1 cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133034
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This study is to analyze genome-wide occupancy of FoxO4 in WT TH1 cells. Naive CD4+ T cells from spleens and lymph nodes were isolated from 8-week-old C57BL/6 mice using a BD FACSAria sorter and cultured in TH1 polarizing condition for 60 hours. ChIPed DNAs were prepared with Active Motif’s ChIP assay kit and subjected to library preparation using NEXTflexTM ChIP-Seq DNA Sequencing Kit. Finally, the DNA libraries were sequenced on an Illumina HiSeq2500 Sequencer.
创建时间:
2020-06-21



