Transcriptome analysis of Citrus reticulata ovule early development based on laser capture microdissection

Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan 'Huagan NO.4' (Citrus reticulata) (MT) and seeded Ponkan 'Egan NO.1' (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion. Overall design: Based on the LCM strategy, we collected 12 samples from three development stages (18 DAP (day after pollening),28 DAP,32 DAP), and two tissues of citrus ovule for RNA-seq. Three biological replicates were designed for each sample. Citrus leaves and ovaries at different development stages were mixed for SMRT sequencing.