Genome-wide DNA accessibility mapping using ATAC-seq in HEK293 in vector control, PC4 knockdown and PC4 phosphorylation specific mutant and mimic

To assess the mechanisms by which PC4 mediates chromatin compaction in cells and regulation of genome organization by its phosphorylation state, we measured the chromatin accessibility landscape using ATAC-seq of vector control 293 cells (Control/shNS), PC4 knockdown (KD/PC4 KD) and upon ectopic expression of Flag tagged PC4 (FP/PC4), Phosphomimic-PC4 (PM/PM-PC4) and phospho-mutant (MTP/MTP5) in PC4 knockdown cells. Overall design: Studying the chromatin accessibility changes that occurred upon knocking down of PC4(PC4 KD) compared to shNS cells and the effect of PC4 phosphorylation on the chromatin accessibility upon expressing Flag tagged PC4, PM-PC4 and MTP5 in the PC4 KD background.