Effects of Tac1, SDF-1α and CXCR4 in BCC entry into BM.

BM: Bone Marrow; PB: Peripheral Blood.
A. Nude mice (n = 20) were injected with 104 BCCs in the mammary fat pad as untransfected, Tac1 knockdown, with SDF-1α and/or CXCR4 expressions. At various times, cells from the central region of femurs (Cellular) and those close to the endosteum (Endosteal) were analyzed by real-time PCR for human genomic β-globin (Figure S1). The results are shown for 72 h time points. B. Analyses similar ‘A’ were done with gDNA from lungs and endosteal regions of the BM.