Single Cell Transcriptomic Analysis of Clinical-used MSCs Derived From Adipose Tissue, Bone Marrow, Placenta Membrane and Umbilical Cord

Here, we use single cell transcriptomics to characterize a dynamic senescence process of in vitro expanded MSC products from adipose tissue (AD), bone marrow (BM), placenta membrane (PM) and umbilical cord (UC). We found that cultured MSCs underwent progressive cell aging. As compared to perinatal MSCs (derived from PM and UC), adult MSCs (derived from AD and BM) showed higher degree of senescence and impaired immunosuppressive function. Moreover, we identified the transcriptional network regulating the intra-population functional diversity, which provides us guidance to drive reprogramming of determined MSCs lineage with specific cellular functions for a particular therapeutic intent. We performed droplet-based scRNA-seq of over 40,000 cells to construct a molecular landscape and dissecting the heterogeneity of MSCs from perinatal and adult human tissues.