miRNAs specifically bound by PrPc during C2C12 cell differentiation

We used RIP-seq to sequenceWT C2C12 and the C2C12 cell line overexpressing WT PrPc to investigate the miRNAs that PrPc in them specifically binds at the fourth day of C2C12 cell differentiation Overall design: To confirm the ability of PrPc to enrich miRNAs, and to analyze the reason why overexpression of PrPc inhibits C2C12 cell differentiation, we used a specific antibody capable of pulling down PrPc, 8H4, against C2C12 cells (Control) and cells stably transfected with PrP (WT PrP) were subjected to formaldehyde cross-linking and RNA immunoprecipitation (RIP) at mid-differentiation Day4 induced with 2% horse serum. By performing miRNA-seq on the enriched RNAs, we identified a large number of selectively enriched miRNAs based on sorted ratios of RIP to input. Displaying the small RNA-seq data for Control and small RNA-seq data for WT PrP and using a log2 fold-change cutoff of 0.5 and a P-value cutoff of 0.05, we defined 31 miRNAs bound by endogenous PrPc in Control, and 41 miRNAs bound by excess PrPc in WT PrP.