HPLC analysis of the end products of ChiA2 treated mucin.
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Reactions were carried out in 20 mM phosphate buffer pH 7.4 with 1 mg/ml rabbit mucin as substrate and incubated at 37°C with 50 µg/ml of pure ChiA2 for 3 hours. The end products were analyzed using a 4.6 nm×250, 5 µm Zorbax carbohydrate analysis column (Agilent Technologies, Waldbrown, Germany) connected to a Shimadzu Prominence 20A; HPLC system. The reaction was stopped by 10% TCA loaded into the HPLC column and eluted using a gradient of 60–80% acetonitrile in water as mobile phase. Each experiment was conducted maintaining same conditions and repeated 3 times.*Not Observed.HPLC analysis of the end products of ChiA2 treated mucin.
创建时间：
2015-12-02
