Bacterial diversity (16S rRNA gene) in participant collected household vacuum dust from homes across two bioclimatic regions (UK and Greece), with associated participant questionnaire and trace element data. (NERC Grant NE/T004401/1)

The <250um fraction of 28 household vacuum dust samples were extracted using high throughput isolation of microbial genomic DNA (21 samples from a national campaign within the UK and 7 samples from Greece, providing samples from two contrasting bioclimatic zones). Both positive and negative reagent controls were included to ensure sterility throughout the processing and sequencing steps, and a randomly selected sample was run in triplicate (DSUK179). These data (raw fastq files: Target_gene 16S and Target_subfragment V4) are available from the European Nucleotide Archive via the study accession PRJEB46920 with individual sample accession numbers ERX6130460 to ERX6130493; https://www.ebi.ac.uk/ena/browser/view/PRJEB46920). A wide range of anthropogenic factors are likely to affect the indoor microbiome and to capture some of this heterogeneity participants were asked to complete a questionnaire. In addition, trace element data were generated using an X-Ray fluorescence spectrometry on the <250um sieved fraction of the household vacuum dust. Sample location data are provided at town/city, Country level. Indoor dust serves as a reservoir for environmental exposure to microbial communities, many of which are benign, some are beneficial, whilst some exhibit pathogenicity. Whilst non-occupational exposure to a range of trace elements and organic contaminants in house dust are a known risk factor for a range of diseases and poor health outcomes, we know far less about the microbial communities associated with our indoor home environments, and their interaction/impacts on human health. Our knowledge of indoor residential bacterial biodiversity, biogeography and their associated drivers are still poorly understood. The data were collected to improve our understanding of the home microbiome.

对来自28个家庭真空尘埃样本的<250微米部分，采用高通量分离微生物基因组DNA的方法进行了提取（其中21个样本来自英国国内的一项国家行动，7个样本来自希腊，从而提供了来自两个对比鲜明的生物气候区的样本）。在整个处理和测序过程中，均包括阳性及阴性试剂对照，以确保无菌。此外，随机选取的一个样本（DSUK179）进行了三重测试。这些数据（原始fastq文件：目标基因16S和目标亚片段V4）可通过欧洲核苷酸档案（European Nucleotide Archive）通过研究访问号PRJEB46920获取，个体样本访问号从ERX6130460至ERX6130493；https://www.ebi.ac.uk/ena/browser/view/PRJEB46920）。多种人为因素可能影响室内微生物群，为了捕捉这种异质性，参与者被要求完成一份问卷。此外，利用X射线荧光光谱法对<250微米筛分出的家庭真空尘埃部分进行了微量元素数据生成。样本位置数据提供了城镇/城市和国家的级别信息。室内尘埃作为环境暴露于微生物群落的储存库，其中许多群落是无害的，一些有益，而一些则表现出致病性。尽管已知非职业性接触家庭尘埃中的多种微量元素和有机污染物是一系列疾病和不良健康结果的已知风险因素，但我们对于与我们的室内家居环境相关的微生物群落及其对人类健康的影响的了解却甚少。我们对室内住宅细菌生物多样性、生物地理及其相关驱动因素的了解仍然十分有限。数据收集的目的是为了增进我们对家庭微生物群的理解。