Hotspot exons are common targets of splicing perturbations

RNA-seq of amiloride-treated cell culture was conducted in order to understand the characteristics that make certain exons susceptible to splicing perturbations. Amiloride is a diuretic that acts by inhibiting epithelial sodium channels in the kidney. It also behaves as a non-specific kinase inhibitor, and has been shown to affect splicing in a handful of genes, presumably by altering phosphorylation of splicing factors. Two samples were sequenced (one untreated, one treated). Differential splicing was done using rMATS version 3.2.5. Poly-A+ RNA from HEK293T cells untreated or treated with amiloride (300 µg/mL) for 6 h was isolated and prepared for RNA-seq.