Detection of S. dysenteriae 1 in 328 stool samples by RDSd1 test versus conventional culture.

1- 22 cultures were positive for S. flexneri, 20 for Vibrio cholerae O1, 5 for Enteroaggregative E. coli, 3 for Salmonella enterica, 2 for Enterotoxigenic E. coli, 2 for Aeromonas spp, 1 for Enteropathogenic E. coli, 1 for Clostridium difficile, 1 for Pseudomonas aeruginosa, 1 for Cryptosporidium parvum. In 52 RDSd1 test-negative samples Shigella spp was not isolated; on 38 of these samples IpaH PCR and Shiga toxin PCR were negative. For technical reasons (insufficient amount of stools, problems of storage), PCR was not done on 14 samples.2- A total of 14 cultures were positive for V. cholerae O1, 1 for S. flexneri, and 1 for Enteroaggregative E. coli.3- 30 stools were positive for parasites (8 Endolimax nana, 6 E. coli, 3 Giardia lamblia, 1 E. histolytica, 1 Trichuris trichiura, 1 Hymenolepis nana, 7 E. nana and E. coli, 1 E. nana and Trichuris trichiura, 1 E. nana and E. histolytica, 1 E. nana and Ascaris lumbricoides).4– During the first epidemic in Boga; 5 Salmonella enterica were identified among the lactose-negative strains received and studied at INRB. During the second epidemic at Ofaï, lactose-negative strains isolated at the dispensary of Bunia and sent to INRB were identified as Salmonella enterica.5- In one of these 4 samples, an Enteroinvasive E. coli and a classical Enteropathogenic E. coli were identified. No enteric pathogen was identified in the other 3 samples that were IpaH PCR negative.6- 1 culture was positive for S. sonnei, 6 were positive for diarrheogenic E.coli (4 Diffusely Adhering E. coli, 1 Enteroinvasive E. coli, 1 with both Diffusely Adhering E. coli and Enteroinvasive E. coli) and 2 for Salmonella enterica.7– No pathogen was identified.