GF colonization with MCJ-KO. MCJ-KO microbiota transplantation to GF mice and DSS-induced colitis

Germ-free C57BL/6 4-6 week old mice were colonized via oral gavage with 200mL of healthy control and MCJ-KO bacterial consortia (50mg-200mg feces). After 2-4 weeks, 2% of DSS was administrated to mice during 6 days followed by two days of water. All gnotobiotic mice were maintained in Techniplast P Isocages and manipulated aseptically for the duration of the experiment. 16S rRNA gene of the V4 region was sequenced from mice feces at day 0 (Before DSS administration) and at day 8 (2 days after DSS administration) to confirm colonization and microbial composition.