PAX3-FOXO1 coordinates enhancer architecture, eRNA transcription, and controls RNA polymerase pause release at select gene targets [ChIP-Seq]

This study aim to in-depth understand the function of a transcription factor fusion protein that drives the development of a highly aggressive pediatric malignancy. After engineered the endogenous PAX3-FOXO1 locus, we use chemical genetics to rapidly degrade the protein. This comprehensive, detailed, and accurate study with deep genomic analyses and deep proteomics provide a detailed mechanism by which PAX3-FOXO1 maintains an oncogenic transcriptional regulatory network. Overall design: Deep mechanistic analysis including proteomics and 15 parameter genomics.