Prolonged Crowding Drives Cancer Cell Invasiveness

Our study found that prolonged crowding drives cancer cell invasiveness with memory retention. To further characterize gene expression regulated by prolonged crowding, RNA sequencing was performed in crowding-primed cells (20 days) followed by sparse culture for different times (0, 1, 3, or 10 days). We examined the duration of the crowding-induced mechanomemory by priming cells for 20 days in crowded or uncrowded culture, followed by sparse culture for 1, 2, 3, or 10 days.