Aflatoxin B₁ in peanut oil from Western Guangdong, China, during 2016–2017

Aflatoxin B₁ (AFB₁) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016–2017 (<i>n</i> = 427) was surveyed. AFB₁ content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg⁻¹. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB₁ was quantified in 47 samples (22.5%) with a range of 15.4–49.9 μg kg⁻¹ in 2016 and in 33 samples (15.1%) with 8.8–22.2 μg kg⁻¹ in 2017, respectively. The AFB₁ contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2–37.9%). Overall, a significant reduction of AFB₁ occurrence was observed in western Guangdong after 2016. It is advisable to control AFB₁ in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary.2016—2017年，研究人员针对粤西地区家庭作坊随机采集的427份花生油样品（n=427），开展了黄曲霉毒素B₁（Aflatoxin B₁, AFB₁）污染情况的调查。采用酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）对样品中的AFB₁含量进行初筛，并通过超高效液相色谱-串联质谱法（UPLC-MS/MS）完成确证分析。该ELISA方法的检出限为1.08 μg·kg⁻¹，回收率介于84.4%~92.6%之间，相对标准偏差为2.2%~4.8%。2016年共有47份样品（占比22.5%）检出AFB₁，含量范围为15.4~49.9 μg·kg⁻¹；2017年则有33份样品（占比15.1%）检出，含量范围为8.8~22.2 μg·kg⁻¹。粤西地区花生油中的AFB₁污染具有季节依赖性，春季为污染最严重的时节，阳性率达24.2%~37.9%。整体而言，2016年后粤西地区AFB₁的污染检出率显著降低。建议对家庭作坊生产的散装及自榨花生油中的AFB₁进行管控，必要时可实施强制性监管措施。