Coccomyxa subellipsoidea C-169: mechanisms of temperature acclimatization

This dataset consists of eight zip archives containing different types of data:TLC: tables with data on phospholipids and galactolipids in thylakoid membranes isolated from C. subellipsoidea cells using TLC metod,Lipids_gravitometry: tables wih data on content of lipids isolated from C. subellipsoidea C-169 cells using Soxhlet apparatus,Proteins_content: tables with data on total protein using the BCA method,Dual_PAM: tables with data of chlorophyll fluorescence of PSII measured with a Dual PAM-100 system (Heinz Walz) equipped with a cooling system, connected to a computer with the DUAL PAM v 1.19,Dual_PAM-FVFM: tables with data of FV/FM measurements with a Dual PAM-100 system,Membrane_fluidity: tables with data of thylakoid membrane fluidity using Cary Eclipse Fluorescence Spectrophotometer,Sugars: data tables containing the amount of soluble sugars in cells C-169,Fatty_acids: tables containing data on the content of saturated and unsaturated fatty acids in C-159 cells.Briefly about the project and the research results obtained:This research aims to clarify the acclimatisation mechanisms that enable the psychrotolerant green alga Coccomyxa subellipsoidea C-169 to grow in a broad temperature spectrum. The contents of various biochemical compounds in cells, the lipid composition of the biological membranes of entire cells, and the thylakoid fraction as well as the electron transport rate and PSII efficiency were investigated. The results demonstrate an acclimatization mechanism that is specific for C. subellipsoidea and that allows the maintenance of appropriate membrane fluidity, for example, in thylakoid membranes. It is achieved almost exclusively by changes within the unsaturated fatty acid pool, like changes from C18:2 into C18:3 and C16:2 into C16:3 or vice versa. This ensures, for example, an effective transport rate through PSII and in consequence a maximum quantum yield of it in cells growing at different temperatures. Furthermore, reactions characteristic for both psychrotolerant and mesophilic microalgae, involving the accumulation of lipids and soluble sugars in cells at temperatures other than optimal, were observed. These findings add substantially to our understanding of the acclimatisation of psychrotolerant organisms to a wide range of temperatures and prove that this process could be accomplished in a species-specific manner.